Dr. Rajan Singh’s research focuses on understanding the role of nitric oxide synthase (NOS)/arginase axis in breast cancer biology. Utilization of L-arginine through NOS or arginase pathway plays an important role in determining the fate of various cell types, including breast cancer cells. Singh’s research seeks to understand the molecular mechanisms by which NOS/arginase pathway regulate cell proliferation, cytostasis or cell death in human breast cancer cells and identify key molecular targets involved in the process. In addition, Singh is interested in the isolation and characterization of human mammary stem cells to study the mechanisms that govern self-renewal and differentiation.
Selected Cancer-Related Publications:
Pervin S, Chaudhuri G, Singh R. NO to breast: when, why and why not? Curr Pharm Des. 2010;16(4):451-62. Review.
Pervin S, Tran AH, Zekavati S, Fukuto JM, Singh R, Chaudhuri G. Increased susceptibility of breast cancer cells to stress mediated inhibition of protein synthesis. Cancer Res. 2008 Jun 15;68(12):4862-74.
Pervin S, Singh R, Hernandez E, Wu G, Chaudhuri G. Nitric oxide in physiologic concentrations targets the translational machinery to increase the proliferation of human breast cancer cells: involvement of mammalian target of rapamycin/eIF4E pathway. Cancer Res. 2007 Jan 1;67(1):289-99.
Singh R, Pervin S, Chaudhuri G. Caspase-8-mediated BID cleavage and release of mitochondrial cytochrome c during Nomega-hydroxy-L-arginine-induced apoptosis in MDA-MB-468 cells. Antagonistic effects of L-ornithine. J Biol Chem. 2002 Oct 4;277(40):37630-6. Epub 2002 Jul 26.
Singh R, Pervin S, Karimi A, Cederbaum S, Chaudhuri G. Arginase activity in human breast cancer cell lines: N(omega)-hydroxy-L-arginine selectively inhibits cell proliferation and induces apoptosis in MDA-MB-468 cells. Cancer Res. 2000 Jun 15;60(12):3305-12.