Dr. Thomas Zangle’s laboratory is applying the principles of fluid mechanics and mass transport to study transport in single cells using both established and emerging microscopy techniques. Current work focuses on the use of live cell interferometry (LCI) to quantify mass distributions within single cells and multicellular clumps by measuring the phase shift of light pasting through single cells to within one nanometer. The resulting measurements can be used to assess the total mass of, or the transport of mass within, individual cells. Work in the Zangle lab explores the use of these techniques as new approaches to study and treat cancer.
Selected Cancer-Related Publications:
Zangle TA, Burnes D, Mathis C, Witte ON, Teitell MA. Quantifying biomass changes of single CD8+ T cells during antigen specific cytotoxicity. PLoS One. 2013 Jul 23;8(7):e68916. doi: 10.1371/journal.pone.0068916. Print 2013.
Zangle TA, Chun J, Zhang J, Reed J, Teitell MA. Quantification of biomass and cell motion in human pluripotent stem cell colonies. Biophys J. 2013 Aug 6;105(3):593-601. doi: 10.1016/j.bpj.2013.06.041.
Chun J, Zangle TA, Kolarova T, Finn RS, Teitell MA, Reed J. Rapidly quantifying drug sensitivity of dispersed and clumped breast cancer cells by mass profiling. Analyst. 2012 Dec 7;137(23):5495-8. doi: 10.1039/c2an36058f. Epub 2012 Oct 11.
Reed J, Chun J, Zangle TA, Kalim S, Hong JS, Pefley SE, Zheng X, Gimzewski JK, Teitell MA. Rapid, massively parallel single-cell drug response measurements via live cell interferometry. Biophys J. 2011 Sep 7;101(5):1025-31. doi: 10.1016/j.bpj.2011.07.022.