Flow Cytometry

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Director: Dr. Zoran Galic

Manager: Ingrid Schmid

Mailing Address
12-953 Factor Building
University of California, Los Angeles
Los Angeles, CA 90095-1745

Telephone: (310) 825-1043
Fax: (310) 794-2145
Email: schmid@mednet.ucla.edu
Website: http://cyto.mednet.ucla.edu

Services

  • Provide instrumentation, as well as technical and professional assistance, for performing laser-based flow cytometry and image flow cytometry
  • Consistent and extensive instrumentation quality control
  • Consultation with the manager, as well as continuous availability of expert advice on flow cytometry during data collection and analysis, through FCSR staff
  • Education through formal courses in basic flow cytometry principles and user operation of flow cytometry analyzers. Classes on nucleic acid flow cytometry and measurement of cell proliferation are also offered

Recharge Rates

There is a tiered fee structure. Costs for JCCC members are listed below; for non-members, please refer to our website for details.

service

JCCC Rate (Hourly)

Sorting $102
Acquisition and analysis by FCSR staff $92
Independent acquisition and analysis $67
LSR II Acquisition and analysis by FCSR staff $99
LSR II independent acquisition and analysis $75
RoboSep Magnetic Cell Separation $60
Basic flow cytometry class (one day) $320 per class
DNA class (half-day) $160

Operating Policies

Faculty investigators, research fellows or others who wish to start using the Flow Cytometry Shared Resource are strongly encouraged to contact the FCSR manager for discussion of their experiments, help with study design and assistance with the procedures and reagents they will need to order. Pilot studies are performed, if necessary. The lab manager remains actively involved in the more complex projects and oversees the technical quality of all work produced by the laboratory. Scheduling is performed through the iLab Management system which can be accessed through the FCSR website. With the exception of developmental work or pilot experiments, the principal investigators are recharged for all time and/or work performed. Details are available on the shared resource website.